Abstract
Bio-based media, derived from cellulosic biomass depolymerisation or bioconversion processes, are composed of several chemicals and biochemicals. In this study, the main components of a hemicellulosic-based medium were analyzed using a dual detection HPLC method to separate and determine concentrations of the major monosaccharides (glucose, xylose, and arabinose), alcoholic and acidic components (ethanol, xylitol, and acetic acid) and furanic compounds (furfural and hydroxymethylfurfural (HMF)). The analyses, which employed a single stationary phase (Aminex HPX-87H) and two detection methods, were carried out under isocratic conditions and involved mobile phases consisting of 5 mM sulfuric acid and acetonitrile in different mix ratios from 0 to 0.061 mole fraction of acetonitrile. Based on the analysis run time and the chromatogram quality, the optimum condition was determined for the simultaneous quantification of the components.
Highlights
Lignocellulosic resources provide a renewable source of carbon mainly (75%) as carbohydrates for the biotransformation-based processes that produce fuels and chemicals for a wide variety of applications [1]
The analytical methods for xylitol determination and quantification could be classified as: 1) methods based on high performance liquid chromatography (HPLC); 2) methods based on gas chromatography (GC); 3) liquid chromatography-mass spectroscopy (LC-MS) methods; and 4) capillary electrophoresis (CE)
Acetonitrile and sulfuric acid were used as the components of the mobile phase in the HPLC analysis, and other materials including glucose, xylose, arabinose, acetic acid, HMF and furfural were used as the standards for the hydrolysate or bioconversion medium analysis
Summary
Lignocellulosic resources provide a renewable source of carbon mainly (75%) as carbohydrates for the biotransformation-based processes that produce fuels and chemicals for a wide variety of applications [1]. The analytical methods for xylitol determination and quantification could be classified as: 1) methods based on high performance liquid chromatography (HPLC); 2) methods based on gas chromatography (GC); 3) liquid chromatography-mass spectroscopy (LC-MS) methods; and 4) capillary electrophoresis (CE). Several kinds of detectors based on various detection methods and chromatographic systems have been used to quantify carbohydrates; these approaches include refractive index (RI) detection, mass spectroscopy (MS), pulsed amperometric detection (PAD), evaporative light scattering (ELS), and ultraviolet (UV) detection. Less sensitive methods such as RI detection and ELS have more applications for xylitol analysis [3,4]
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