Simultaneous Quantification and Pharmacokinetic Study of Nine Bioactive Components of Orthosiphon stamineus Benth. Extract in Rat Plasma by UHPLC-MS/MS.

Guo Guo,Liang Liang,Zhu Zhu,Li Li,Bai Bai,Su Su,Xie Xie,Gu Gu

doi:10.3390/molecules24173057

Guo Guo, Liang Liang + Show 6 more

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https://doi.org/10.3390/molecules24173057

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Abstract

Orthosiphon stamineus Benth. (OS) is a traditional folk medicine for the treatment of kidney stones and other urinary tract diseases. In this study, a rapid and sensitive Ultra high-performance liquid chromatography (UHPLC)-MS/MS approach was established and validated for the simultaneous quantification of nine bioactive components in rat plasma. The nine components from OS extract detected in rat plasma were danshensu, protocatechuic acid, caffeic acid, rosmarinic acid, salvianolic acid A, salvianolic acid B, cichoric acid, sinensetin and eupatorin. After liquid-liquid extraction with ethyl acetate, the plasma samples were subjected to a triple quadrupole mass spectrometer employing electrospray ionization (ESI) technique and operating in multiple reaction monitoring (MRM) with both positive and negative ion modes. The standard curves showed good linear regression (r > 0.9915) over the concentration range for the nine analytes. The inter-day and intra-day precision and accuracy were found to be within 15% of the nominal concentration. The recovery and stability of nine compounds were all demonstrated to be within acceptable limits. The approach was successfully applied to investigate the pharmacokinetic analysis of the nine bioactive components after oral administration of OS extract in rats.

Highlights

Natural products have been an exemplary source of new drugs, and many of the currently available medicines have been directly or indirectly derived from them [1]
The mobile phase played a key role in the acquisition of good chromatographic behavior and appropriate ionization
To obtain good peak shape and high detection sensitivity, different amounts of formic acid and acetic acid were added to the aqueous phases

Summary

Introduction

Natural products have been an exemplary source of new drugs, and many of the currently available medicines have been directly or indirectly derived from them [1]. The dried whole plant of OS, named “Shen Cha”, is a traditional folk medicine for the treatment of kidney stone and other urinary tract diseases [2]. OS contains several bioactive constituents, such as terpenoids and phenolic compounds, but one of the most important classes of the bioactive constituents is the phenolic group [3]. The reported therapeutic effects of OS, such as anti-inflammatory [4], diuretic [5], hypouricemic [2], antidiabetic [6], anti-hypertensive [7], anti-oxidant [8], hepatoprotective [9] and antiproliferative [10] activities ascribed mainly to the phenolic compounds. Protocatechuic acid (PCA), danshensu (DSS), salvianolic acid A (Sal A)

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