Abstract
A high performance liquid chromatographic method coupling with electrospray ionization mass spectrometry (HPLC/ESI-MS) for simultaneous determination of phenformin, rosiglitazone, glibenclamide and glimepiride in dietary supplements and traditional Chinese medicines for diabetes mellitus was proposed. The separation was achieved on a C 18 column with the mobile phase consisted of acetonitrile and water (0.05% formic acid (v/v) and 20 mM ammonium acetate), at a flow rate of 1.0 ml/min with gradient elution. The analytes were identified and quantified by ESI-MS. Sildenafil was selected as internal standard. Full validation of the proposed method was provided (selectivity, linearity, limit of detection, limit of quantification, precision and accuracy). In contrast to existing methods, the proposed method has obvious advantages of simplicity, rapidity, accuracy and good applicability, and it has been applied to analyze illegal additives in nine dietary supplements and eight traditional Chinese medicines successfully.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
