Simultaneous nitriles degradation and bioflocculant production by immobilized K. oxytoca strain in a continuous flow reactor

Abstract

High cost is one of the limiting factors in the industrial production of bioflocculant. Simultaneous preparation of bioflocculant from the contaminants in wastewater was considered as a potential approach to reduce the production cost. In this study, butyronitrile and succinonitrile were verified as sole nitrogen sources for the growth of strain K. oxytoca GS-4-08 in batch experiments. Moreover, more than 90 % of the mixed nitriles could be degraded in a continuous flow reactor, and the bioflocculant could be prepared simultaneously in the effluent. All the as-prepared bioflocculants exhibited high flocculation efficiencies of over 90 % toward Kaolin solution. FTIR and XPS results further unveiled that, the bioflocculant samples with abundance of carboxyl, amine and hydroxyl groups may play an important role on adsorption of Pd2+. The adsorption process could be well simulated by Freundlich model, and the Kf values were as high as 452.8 mg1−1/n l1/n g-1. The results obtained in this study not only confirm the technical feasibility for preparation of bioflocculant from various single nitrile and/or mixed nitriles, but also promise its economic feasibility.