Abstract
To enhance the selectivity of liquid chromatographic—electrochemical assays for biogenic amines and metabolites in brain tissue, multiple electrode transducers were investigated. Two configurations of dual working electrodes were examined: parallel—adjacent and series arrangements. Using the raw detector currents with each configuration, peak-height ratios from simultaneously generated chromatograms were calculated to assess the selectivity of the instrument for direct injections of brain tissue supernatant. Ratios were consistent with injections of standards. Nearly coeluting peaks such as norepinephrine and 3-methoxy-4-hydroxyphenylglycol were resolved by using dual detector electrodes in series; only the catecholamines were detected at the downstream electrode owing to their electrochemical reversibility. The scheme was applicable to the assay of norepinephrine, 3,4-dihydroxyphenylacetic acid, dopamine, homovanillic acid, serotonin, 5-hydroxytryptophan and 5-hydroxyindole-3-acetic acid in brain tissue in less than 15 min.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
