Abstract
We present a method that enables us to construct fluorescence fingerprints from samples, with respect both to wavelength- and to time-dependences. A Xe - Cl excimer laser is used for excitation of fluorescing compounds. For every fluorophore, the time course of the fluorescence is recorded over time, as a function of the emission wavelength, subsequent to photoexcitation. Time-resolved multispectral analysis is performed. We derive a linear spectro-temporal kernel: a weighting function, which describes the temporal behaviour of the fluorescence spectrum within the time-bandwidth imposed by our sampling interval (Nyquist frequency), independently of the pulse width of the excitation . The data analysis revealed both the static and the dynamic fluorescence properties of the fluorophores under study.
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