Abstract
Microwave extraction and separation has been used to increase the concentration of the extract compared to the conventional method with the same solid/liquid ratio, reducing extraction time and separate at the same time Volatile Organic Compounds (VOC) from non-Volatile Organic Compounds (NVOC) of boldo leaves. As preliminary study, a response surface method has been used to optimize the extraction of soluble material and the separation of VOC from the plant in laboratory scale. The results from the statistical analysis revealed that the optimized conditions were: microwave power 200 W, extraction time 56 min and solid liquid ratio of 7.5% of plants in water. Lab scale optimized microwave method is compared to conventional distillation, and requires a power/mass ratio of 0.4 W/g of water engaged. This power/mass ratio is kept in order to upscale from lab to pilot plant.
Highlights
Boldo (Peumus boldus Molina) is a dioic plant endemic in Chile [1]
The high performance thin layer chromatography (HPTLC) analysis of boldine served as a marker of interesting non-Volatile Organic Compounds (NVOC) extracted; gas chromatography coupled to mass spectrometry (GC-MS) was used to identify and to have the relative proportion of each molecules in Volatile Organic Compounds (VOC) extracted; to help us monitor the efficiency of the up scaling
ANOVA data summarized in a Pareto chart in Figure 2 for NVOC yield represents the significant effects of all variables and their interactions
Summary
Boldo (Peumus boldus Molina) is a dioic plant endemic in Chile [1]. Several pharmacopeias document its uses as leaves infusions for notably digestives and hepato-biliairy protective effects. Boldo leaves contain 0.4%–3% of essential oil, mainly composed of VOC, such as terpenes (ascaridole, p-cymene, d-limonene...) These molecules can have a low solubility in water, giving aqueous extract a distinctive odor. Microwave assisted extraction (MAE) is a reliable alternative method of extraction Compared to hydrodistillation, it consumes less energy and requires less time to carry out the extraction of essential oil [13,14]. It consumes less energy and requires less time to carry out the extraction of essential oil [13,14] We applied this microwave method in order to extract the different compounds of the boldo leaves in a single step, and to accelerate the hydrodistillation. The high performance thin layer chromatography (HPTLC) analysis of boldine served as a marker of interesting NVOC extracted; gas chromatography coupled to mass spectrometry (GC-MS) was used to identify and to have the relative proportion of each molecules in VOC extracted; to help us monitor the efficiency of the up scaling
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