Abstract
Objective: The current investigation was pointed at completely unique and reliable high performance liquid chromatographic method for simultaneous quantification of Choline salicylate and Tannic acid. Methods: Chromatographic separation was achieved on a symmetry C18 column (150x4.6mm, 3.5 µ) using isocratic elution with a buffer containing 1 ml of OPA in l lt of HPLC marked water and acetonitrile within the percentage of 60:40 as movable phase with a flow rate of 1.0 ml/min at ambient temperature. Analysis was achieved within 15 min over an honest linearity within the concentration range from 4-60 µg/ml of choline salicylate and 2.5-37.5 µg/ml of tannic acid. Stress conditions of degradation in acidic, alkaline, peroxide and thermal was studied. Results: LOD and LOQ were observed as 1.21 µg/ml, 0.758 µg/ml and 4 µg/ml, 2.5 µg/ml of choline salicylate and tannic acid respectively. Precision and recovery study results were found to be within the suitable limit. Conclusion: This developed method showed reliable, precise, accurate results under optimized conditions. The method was validated as reported by ICH guidelines. Hence it was evident that the proposed method was suitable for regular analysis and quality control of pharmaceutical preparations.
Highlights
Choline salicylate is used as a pain reliever
The current study was designed to develop a simple, precise and rapid analytical RP-HPLC method, which can be used for the analysis of assay method for simultaneous estimation of choline salicylate and tannic acid in bulk and pharmaceutical dosage form
Various combinations were tried for the separation of choline salicylate and tannic acid
Summary
Choline salicylate is used as a pain reliever. It is an anti-inflammatory [1] drug-related to aspirin. Choline salicylate is used to treat arthritis and used to reduce swelling and mild-moderate pain. This drug is used to treat fever [2, 3]. It reduces mild to moderate pain, fever and inflammation or swelling. Choline salicylate is powerful in the treatment of gout [7, 8], rheumatic fever [9, 10], rheumatoid arthritis [11, 12] and muscle injuries. To relieve pains associated with tooth growth in infant population, choline salicylate is used as main ingredient in teething gels.
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