Abstract
A simple, accurate and precise HPTLC method was developed and validated for simultaneous estimation of 1, 8-Cineole and Betulinic acid in Shati Vati - An Ayurvedic formulation by HPTLC. Shati Vati contains Shati rhizome powder have 1-8 cineole and Badar ripe fruit powder have Betulinic acid reported medicinal claims and uses. In the analytical method, toluene: ethyl acetate (93:07 v/v) was finalized as the mobile phase and a clear separation was achieved. The wavelength for 1-8 cineole and Betulinic acid was 665nm and 580nm respectively. The Rf value of 1-8 cineole and betulinic acid was obtained 0.647 and 0.213 respectively. The developed analytical method was validated for Linearity, Accuracy, Precision, LOD, LOQ and Robustness as per ICH Q2 (R1) guideline. Results for the validation process were within the range of acceptance criteria of ICH guideline. HPTLC method have been applied successfully for estimation of 1-8 Cineole and Betulinic acid in Shati Vati an ayurvedic formulation.
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