Abstract
The intracellular free Ca 2+ concentration ([Ca 2+] i) was measured simultaneously with the contractile response in rate mesangial cells by using a dedicated apparatus. The registration of the [Ca 2+] i transient was based on the spectroscopic determination of the free and Ca 2+-bound indo-1 concentration, whereas the cell volume was obtained by measuring the transmitted light intensity at a wavelength which was not absorbed by cellular components and by relating the calculated absrobance to the cell volume. Long-term pretreatment of mesangial cells with human interleukin-1β (24 h; nM) increased the angiotensin II induced mobilization of [Ca 2+] i and led to a reduced, but sustained contraction of the cells. The control cells contracted faster and stronger as compared to IL-1β-pretreated cells. Six seconds following the increase in Ca 2+ i, the cells started to contract. Pretreatment with IL-1β had no significant effect on the time lag between the Ca 2+ release and the induction of the contractile response. The simultaneous registration of Ca 2+ transients and cell volumes allows new insights into the sequence and dynamic nature of pharmcologically-induced biological processes.
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