Abstract

Three ruminal-cannulated Holstein cows in early lactation were fed three diets, each containing different energy and protein supplements in two consecutive 3 X 3 Latin squares. Each supplement contained a combination of three different feedstuffs (ground corn, soybean meal and wheat mids; corn gluten feed, rolled oats and distiller's dried grains; ground barley, brewer's grains and cottonseed meal). Diets consisted of 30% corn silage, 10% alfalfa hay, 10% alfalfa haylage, 17.7% ground corn plus minerals and vitamins, with the remainder as supplemental feedstuffs individually marked for measurement of ruminal turnover and in situ digestion rates. An extra period at the end of each Latin square was used to measure ruminal turnover and in situ digestion of individual forages. Diet had no effect on dry matter intake or milk yield. Mean fractional turnover (per hour) rate, measured by labeling feedstuffs with cerium, samarium or lanthanum was .044, .048, .049, .043, .047, .046, .050, .047, .049, .037, .046 and .045 for corn, soybean meal, wheat mids, corn gluten feed, oats, distiller's dried grains with solubles, barley, brewer's dried grains, cottonseed meal, alfalfa hay, corn silage and alfalfa haylage (P greater than .1), respectively. In situ-predicted ruminal degradation of N weighted for rate of passage was 61.4, 67.0, 81.5, 74.8, 86.3, 71.0, 75.7, 52.1, 54.2, 60.8, 71.7 and 70.9% for respective feedstuffs (P less than .05). In comparison with mean literature values for in vivo-measured N degradability, mean literature value = 1.172 (in situ predicted) -9.73 (P less than .05, R2 = .51). Results are interpreted to indicate a tendency for overestimating ruminal N degradability by in situ methods in feedstuffs of low degradability, while underestimating degradability in more highly degraded feedstuffs. Estimates were 11 to 17 percentage units lower than literature values for alfalfa hay and haylage and 17 units lower than literature values for distiller's dried grains.

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