Abstract
A simple and rapid method for simultaneous measurements of 13C/ 12C and 15N/ 14N ratios and organic C and N contents in biological samples at microgram levels by coupling of QMS and modified CHN elemental analyzer was examined for the use of stable carbon and nitrogen isotope as a tracer. Monitoring of CO 2(m/z=44 and 45) and N 2(m/z=28 and 29) ion currents due to the combustion product gas by the elemental analyzer allows simultaneous measurements of both isotope ratios within 0.5 % at 10–500 microgram. Organic C and N contents were determined within 5–12 % relative standard deviation at the same levels, depending upon the combustion conditions.
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