Abstract
Blood flow directly affects nutrient and oxygen as well drug delivery in tumors. The distribution of blood within tumor is disturbed by structural and functional abnormalities, and may be further altered by therapy. In turn, this results in heterogeneous distribution of drugs and/or oxygen, with direct effects on the efficacy of cytotoxic therapies. These heterogeneities are poorly understood because of the inability of current techniques to characterize the function of individual vessels and their local network. To address this problem, we have developed a novel methodology for simultaneously quantifying blood flow (velocity, flux, hematocrit and shear rate) in extended networks at the single capillary level. Our approach relies on deconvolution of signals produced by labeled red blood cells as they move relative to the scanning laser of a confocal or multiphoton microscope, and provides fully‐resolved three‐dimensional flow profiles within vessel networks. Using this methodology, we show that blood velocity profiles are perturbed and asymmetrical near intussusceptive tissue structures in tumors. Furthermore, we show that subpopulations of vessels, classified by functional parameters, exist in different tissue regions, and that Angiopeitin‐1 can improve tumor perfusion by inducing arteriogenesis.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.