Abstract
Recently, non-invasive, real-time and multi-point measurement of neural activities has become possible by using a multi-electrode array (MEA). Another method for multi-point measurement is the fluorescent imaging technique using voltage indicator dyes or calcium indicator dyes. Especially, calcium imaging using fluorescent calcium indicator dyes is often more useful, because they exhibit larger changes in the fluorescence intensity than voltage indicator dyes and their fluorescence changes can be detect easily. Additionally, calcium signals play key roles in the brain function, such as the long-term potentiation (LTP) in the hippocampus, and calcium imaging can be a powerful tool to elucidate the brain function. In this study, we constructed a measurement apparatus combining the MEA system and laser confocal calcium imaging and simultaneously measured electric signals and calcium signals in acute mouse hippocampal slices. The obtained results showed the availability of the present method.
Highlights
IntroductionThe brain function is based on complicated interactions among electric neural activities, intracellular calcium signals, intercellular communications by neuro-
For the long-term potentiation (LTP) measurement in the hippocampus, for example, electric stimulation such as the tetanic stimulation or theta burst stimulation is applied by a microelectrode inserted around the Shaffer collateral, and field excitatory postsynaptic potential is extracellularly measured by another microelectrode inserted in the CA1 region
The electric signals were measured in the whole of hippocampus (Figure 3(a) and Figure 3(c)) and the calcium signals were measured in the CA1 region (Figure 3(a) and Figure 3(b)) which is shown with blue rectangles in Figure 3(a) and Figure 3(c)
Summary
The brain function is based on complicated interactions among electric neural activities, intracellular calcium signals, intercellular communications by neuro-. The long-term potentiation (LTP), a persistent increase in synaptic strength following high-frequency stimulation of a chemical synapse, is a widely accepted cellular model for learning and memory, and an activity-dependent intracellular Ca2+ increase represents a key signal for the activation of the mechanism. Electric signals in the brain can be measured by the conventional electrophysiological methods. For the LTP measurement in the hippocampus, for example, electric stimulation such as the tetanic stimulation or theta burst stimulation is applied by a microelectrode inserted around the Shaffer collateral, and field excitatory postsynaptic potential (fEPSP) is extracellularly measured by another microelectrode inserted in the CA1 region
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