Abstract
An assay for simultaneous measurement of glutathione and ascorbic acid, two mutually related important aqueous antioxidants in the biological matrix, is reported. This assay involved an HPLC system equipped with an electrochemical detector and Au/Hg electrode. A microdialysis perfusion system was coupled with this LC-ECD system for on-line and continuous determination of glutathione and ascorbic acid levels in extracellular fluids of anesthetized rat liver. Good reproducibility for both ascorbic acid and glutathione was achieved when standard glutathione and ascorbic acid solutions were used. Additionally, the effects of global liver ischemia on the hepatic extracellular ascorbic acid and glutathione levels were investigated. The simultaneous monitoring of ascorbic acid and glutathione levels is important since glutathione and ascorbic acid are mutually related in many biological functions.
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