Simultaneous Measurement of 67 Non-Human Primate Immunological, Metabolic and Hormonal Biomarkers using Luminex® xMAP® Technology

Abstract

Abstract Immunology, metabolic, and pituitary biomarker research in non-human primates (NHP) is essential for the study of several diseases and biological regulation. To meet this need, we developed three NHP MILLIPLEX® multiplex panels: 12-plex NHP Metabolic Hormone, 7-plex NHP Pituitary Panel 1, and our new 48-plex NHP Cytokine/Chemokine Panel A for the simultaneous measurement of picogram levels of 67 immune, metabolic and hormone factors, in small volumes of serum, plasma or cell culture samples using Luminex® xMAP® technology. Serum from in vivo LPS-challenged animals (n=2 cynomolgus, n=2 rhesus) demonstrated increased levels of 19 cytokines/chemokines with peak elevated levels at 1 hr for 4 analytes (e.g. IL-10, TNFα), at 3 hr for 11 analytes (e.g. IL-6, IFNγ) and at 8 hr (e.g. Fractalkine, I-TAC). Metabolic markers, GIP and GLP-1 (active) in rhesus, also increased significantly at 8 hr and 3 hr, respectively (p<0.02). Significant differences between fasted and fed serum sample values (n=5 cynomolgus, n=5 rhesus) were found for several metabolic markers, including C-peptide, GIP, Insulin and PP, which increased with feeding (P<0.05). In cynomolgus, I-TAC and IP-10 significantly increased after feeding (P<0.003). Testing healthy serum samples from three additional NHP species (African green monkey (n=5), baboon (n=5), chimpanzee (n=5)) exhibited overall sample detectability of 89%, 77%, and 64% for the MILLIPLEX®Cytokine/Chemokine Panel A, Pituitary, and Metabolic multiplex panels, respectively. Thus, the MILLIPLEX®NHP Metabolic Hormone, NHP Pituitary Panel 1 and our latest and largest NHP panel, the 48-plex NHP Cytokine/Chemokine Panel A, are valuable tools for profiling 67 biomarkers in 5 NHP species for disease research.