Abstract
The value of ultra-thin polyacrylamide gels (0·15mm) for the simultaneous separation of phosphoglucomutase (PGM 1) and erythrocyte acid phosphatase (EAP) by isoelectric focusing of human red cell lysates and bloodstain extracts is investigated. Simultaneous typing of the PGM 1 and EAP systems in a pH 5-pH8 gradient is possible without the introduction of an additional ampholine range. The cost per plate is reduced from approximately £2.80 (ampholines only) when using a l mm thick gel to only £0·70 for the 0·15mm system.
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