Simultaneous integration of biosensor and EEG measurements in freely moving rodents: Dynamic changes of brain glucose and glutamate concentrations during transitions between different sleep states in the mouse pre-frontal cortex

Abstract

Event Abstract Back to Event Simultaneous integration of biosensor and EEG measurements in freely moving rodents: Dynamic changes of brain glucose and glutamate concentrations during transitions between different sleep states in the mouse pre-frontal cortex David A Johnson1, Daniel V. Aillon1*, Erik Naylor1, Hans Harmon1 and George S. Wilson2 1 Pinnacle Technology Inc., United States 2 University of Kansas, United States This work demonstrates a direct correlation between glutamate and glucose levels in the pre-frontal cortex of mice and sleep state. Male C57 mice were simultaneously implanted with an intracerebral guide cannula targeting the prefrontal cortex and electrodes to measure cortical electroencephalographs (EEG). A turn-key Pinnacle 8401 EEG/EMG/Biosensor system was used to record real-time, second by second measurements of glucose or glutamate levels and EEG activity over a 24 hour period. Results showed that glucose levels in mice transitioning from waking to slow-wave sleep rose an average of 116 +/- 27 uM (n=57 episodes). Conversely, glucose levels in mice transitioning from sleep to wakefulness resulted in an average decrease of 121 +/- 27uM glucose. (n=54 episodes). Glutamate levels rose an average of 0.52 +/- 0.21uM upon during REM episodes lasting longer than one minute before returning to baseline levels following the REM episode. Second by second measurement of neurochemicals such as glutamate, glucose and lactate in the brain using electrochemical biosensors is a powerful tool that can be used to profile concentration changes due to pharmacological and behavioral interactions. Funding through NIH SBIR Grant #5T44MH076318-03 Conference: 41st European Brain and Behaviour Society Meeting, Rhodes Island, Greece, 13 Sep - 18 Sep, 2009. Presentation Type: Poster Presentation Topic: Poster presentations Citation: Johnson D, Aillon DV, Naylor E, Harmon H and Wilson GS (2009). Simultaneous integration of biosensor and EEG measurements in freely moving rodents: Dynamic changes of brain glucose and glutamate concentrations during transitions between different sleep states in the mouse pre-frontal cortex. Conference Abstract: 41st European Brain and Behaviour Society Meeting. doi: 10.3389/conf.neuro.08.2009.09.183 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 10 Jun 2009; Published Online: 10 Jun 2009. * Correspondence: Daniel V Aillon, Pinnacle Technology Inc., Le Locle, United States, aillond@pinnaclet.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers David A Johnson Daniel V Aillon Erik Naylor Hans Harmon George S Wilson Google David A Johnson Daniel V Aillon Erik Naylor Hans Harmon George S Wilson Google Scholar David A Johnson Daniel V Aillon Erik Naylor Hans Harmon George S Wilson PubMed David A Johnson Daniel V Aillon Erik Naylor Hans Harmon George S Wilson Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.