Abstract
This work involved the optimization and validation of a quantitative HPLC-MS/MS method to determine 38 hormonally growth promoter residues in bovine muscle, among them steroids, stilbenes, and resorcylic acid lactones. The enzymatic hydrolysis process using β-glucuronidase/aryl sulfatase enzyme derived from Helix Pomatia juice was studied through a 23 factorial design, in which the chosen conditions were to perform the reaction using 50 μL of enzyme at 37.5 °C for 16 h. The validation procedures were executed in accordance with the Decision 657/2002/EC guidelines. A survey study was performed in which 80 samples originated from Brazilian slaughterhouses and imports from third countries were analyzed, and 17β-estradiol, 17α-testosterone, 17β-testosterone, and progesterone were detected in greater concentrations than the respective CCα values. The developed method has been accredited in ISO/IEC 17025 and applicable in regulatory analysis.
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