Simultaneous-HPLC Quantification of Phenolic Acids in Traditionally used Ayurvedic Herb Diplocyclos palmatus (L.) Jeffry

Ankita Misra,Sharad Srivastava,Aks Rawat,Bhanu Kumar,Abhishek Niranjan,Pushpendra Kumar Shukla

doi:10.5530/pj.2017.4.78

Abstract

Introduction:The present study deals with the simultaneous HPLC-quantification of phenolic acid(s) in the aerial parts of Diplocyclos palmatus (Cucurbitaceae) and evaluation of their bioactivity potential through in vitro antioxidant assay’s. Method: The HPLC elution was done using C18 column using gradient (binary phases) solvent system at a flow rate of 0.6 ml/min. Total phenolic and, flavonoid contents were determined and the antioxidant potential was estimated by four assay’s viz. DPPH radical scavenging assay, ferric reducing power assay, total antioxidant capacity and 2-deoxy ribose assay. Results: The species is rich in three phenolic acids, among which gallic acid (1708 ug/g) is in maximum concentration followed by caeffic acid (437 ug/g) and protocateuchic acid (337.7 ug/g). Total phenolic content was higher (10.5 mg/g) than flavonoid content (3.78 mg/g) and TAC was found at 0.137 mg/g ASE (ascorbic acid equivalent). IC50 of D. palmatus extract for scavenging of hydroxyl radical by 2-deoxy ribose and DPPH was at concentration of 125.61 ± 0.834 (μg/ml) and 353.71 ± 0.663 (μg/ml) respectively. In vitro antidiabetiv potential, via inhibition of alpha amylase enzyme through starch iodine and 3,5- DNS assay reveals the IC50 of extract at 146.31 ± 0.415 ug/ml and 286.23 ± 0.671 ug/ ml respectively. Conclusion: The species (aerial part) was rich in phenolic acid with potential bioactivity, identified leads will be useful in further chemical characterization and pharmacological validation.

Highlights

The present study deals with the simultaneous HPLC-quantification of phenolic acid(s) in the aerial parts of Diplocyclos palmatus (Cucurbitaceae) and evaluation of their bioactivity potential through in vitro antioxidant assay’s
A review of literature on the traditional knowledge about D. palmatus reveals that the herb is conventionally used since long ago among tribals and rural people as a potential remedy for many reproductive disorders but due to lack of considerable focus on healthcare system of such communities, this highly valuable herb is losing its charm
Among the identified markers gallic acid (1708 ug/g), was found in heighest concentration followed by caeffic acid (437 ug/g) and protocateuchic acid (337.7 ug/g) as shown Table 1

Summary

Introduction

The present study deals with the simultaneous HPLC-quantification of phenolic acid(s) in the aerial parts of Diplocyclos palmatus (Cucurbitaceae) and evaluation of their bioactivity potential through in vitro antioxidant assay’s. The species (aerial part) was rich in phenolic acid with potential bioactivity, identified leads will be usefull in further chemical characterization and pharmacological validation. The plant is known to have alkaloids, saponins, phenols, steroids and cardiac glycosides etc.[8] In vitro studies revealed that D. palmatus (aerial part) contain antioxidant and anti-microbial activity.[9,10] Seeds have analgesic, anti-inflammatory and antiarthritic activity.[11] In vivo anticonvulsant activity of leaves were studied and chloroform extract was found to be more potential than aqueous and alcoholic extracts in controlling both MES and PTZ convulsions.[12]

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Results

Conclusion