Abstract
An HPLC method for the simultaneous quantitative determination of betamethasone and clotrimazole in cream formulation has been developed. The method utilizes a reversed-phase C18 (250 X 4.0 mm) stationary phase, with a mixture of methanol-acetate buffer-acetonitrile (33:27:40, v/v) as a mobile phase, and spectrophotometric UV detection at 254 nm. The method has been validated for cream formulations containing betamethasone and clotrimazole with linear range of 0.025 to 0.075 mg/ml for betamethasone with a correlation coefficient of 0.9996, and linear range of 0.25 to 0.75 mg/ml for clotrimazole with a correlation coefficient of 1.000. The results demonstrated that this method is accurate, precise, specific, linear, reliable, sensitive, and fast.
Highlights
Betamethasone is a potent synthetic glucocorticoid that is widely used for the treatment of inflammation, allergies and other diseases related to glucocorticoid deficiency [1]
Betamethasone has been determined in different pharmaceutical preparations by HPLC [1,67]
Clotrimazole has been determined in different pharmaceutical preparations by: Titration method [2], gas liquid chromatography [8], high performance TLC (HPTLC) [9], micellar electrokinetic chromatography (MEKC) [10] and by HPLC [11,12,13,14]
Summary
Betamethasone is a potent synthetic glucocorticoid that is widely used for the treatment of inflammation, allergies and other diseases related to glucocorticoid deficiency [1]. Betamethasone has been determined in different pharmaceutical preparations by HPLC [1,67]. Clotrimazole has been determined in different pharmaceutical preparations by: Titration method [2], gas liquid chromatography [8], high performance TLC (HPTLC) [9], micellar electrokinetic chromatography (MEKC) [10] and by HPLC [11,12,13,14]. Reversed-phase LC for the simultaneous determination of betamethasone and clotrimazole in cream formulations has been described in the USP [15]. The main objective of this study is, to develop and validate an HPLC method involving minimum sample preparation, good resolution, reasonable analysis time, good accuracy, high precision, good specificity, good linearity, and excellent reliability
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