Abstract
Rhodococcus sp. CPZ24 was isolated from swine wastewater and identified. Batch (0.25L flask) experiments of nitrogen removal under aerobic growth conditions showed complete removal of 50mgL−1 ammonium nitrogen within 20h, while nitrate nitrogen removal reached 67%. A bioreactor (50L) was used to further assess the heterotrophic nitrification and aerobic denitrification abilities of Rhodococcus sp. CPZ24. The results showed that 85% of the ammonium nitrogen (100mgL−1) was transformed to nitrification products (NO3--N and NO2--N) (13%), intracellular nitrogen (24%), and gaseous denitrification products (48%) within 25h. The ammonium nitrogen removal rate was 3.4mg L−1h−1. The results indicate that the strain CPZ24 carries out simultaneous nitrification and denitrification, demonstrating a potential use of the strain for wastewater treatment.
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