Abstract
The acetyl-CoA carboxylase-α (ACACA) gene is the rate-limiting enzyme for the biosynthesis of fatty acids in animal tissues. By sequencing analysis in promoter III of the ovine ACACA we identified 2 novel SNPs (A>G at 1168bp and a T nucleotide insertion at 1182bp) (GenBank accession number AJ292286). The novel variant (A>G at 1168bp) was present in Cigaja, Sumavka, and Improved Valachian breed kept in the Czech Republic and T insertion at 1182bp was present in the East Friesian breed. A protocol for rapid efficient simultaneous genotyping of ACACA variants using the primer extension method (PEA) was described, and a total of 171 animals belonging to sheep breeds: Cigaja (9), Sumavka (87), East Friesian (59) and Improved Valachian (16) were genotyped. Variant alleles are: A/G at 1168bp, G/T at 1330bp, C/G at 1138bp and C/T at 1430bp.The genotype combination AG/GT/CG/CC occurred in all 4 breeds kept in the Czech Republic with frequency of occurrence ranging from 15.25% to 44.45%. Allele frequencies of the Cigaja breed differ significantly from the other Czech sheep breeds.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
