Abstract
Currently, endometrial carcinoma (EC) is the most common genital cancer in high-income countries. Some types of endometrial hyperplasia (EH) may be progressing to this malignancy. The diagnosis of EC and EH is based on time consuming histopathology evaluation, which is subjective and causes discrepancies in reassessment. Therefore, there is a need to create methods of objective evaluation allowing the diagnosis of early changes. The study aimed to simultaneously asses Fourier Transform Infrared (FTIR) and Raman spectroscopy combined with multidimensional analysis to identify the tissues of endometrial cancer, atypical hyperplasia and the normal control group, and differentiate them. The results of FTIR and Raman spectroscopy revealed quantitative and qualitative changes in the nucleic acid and protein in the groups of cancer and atypical hyperplasia, in comparison with the control group. Changes in the lipid region were also observed in Raman spectra. Pearson correlation coefficient demonstrated a statistically significant correlation between Raman spectra for the cancer and atypical hyperplasia groups (0.747, p < 0.05) and for atypical hyperplasia and the controls (0.507, p < 0.05), while FTIR spectra demonstrated a statistically significant positive correlation for the same group as in Raman data and for the control and cancer groups (0.966, p < 0.05). To summarize, the method of spectroscopy enables differentiation of atypical hyperplasia and endometrial cancer tissues from the physiological endometrial tissue.
Highlights
The endometrium lining of the uterine cavity is a structure subjected to changes in the menstrual cycle or pregnancy, as well as during such periods of a woman’s life as puberty and menopause.Currently, endometrium becomes the subject of scientific interest due to the growing incidence of endometrial cancer in developed countries [1].Endometrial hyperplasia is of particular interest since certain types of this hyperplasia may predispose to endometrial cancer
To the best of our knowledge, this study is the first to use both Fourier Transform Infrared and Raman spectroscopy combined with multidimensional analysis to identify and differentiate tissues of endometrial cancer, atypical hyperplasia and the normal control group
The obtained results showed, that in comparison with the control group, the most visible structural changes in the non-control groups, which were observed as shift or absence of peaks, were noticed in the Raman and Fourier Transform Infrared (FTIR) regions originating from deoxyribonucleic acid (DNA), RNA and proteins
Summary
The endometrium lining of the uterine cavity is a structure subjected to changes in the menstrual cycle or pregnancy, as well as during such periods of a woman’s life as puberty and menopause.Currently, endometrium becomes the subject of scientific interest due to the growing incidence of endometrial cancer in developed countries [1].Endometrial hyperplasia is of particular interest since certain types of this hyperplasia may predispose to endometrial cancer. The endometrium lining of the uterine cavity is a structure subjected to changes in the menstrual cycle or pregnancy, as well as during such periods of a woman’s life as puberty and menopause. Endometrium becomes the subject of scientific interest due to the growing incidence of endometrial cancer in developed countries [1]. There are an estimated 200,000 new cases of endometrial hyperplasia per annum [2]. Simple and complex hyperplasia without atypia are not considered risk factors for endometrial cancer, whereas forms of hyperplasia with cytological atypia pose such a risk. The cases of endometrial cancer include type I cancer preceded by estrogen-stimulated hyperplasia and type II with a different mechanism of formation [3,4]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
