Abstract
A simple and rapid method for the simultaneous extraction and determination of sulfadiazine and trimethoprim in medicated fish feed by HPLC using sulfadimidine as internal standard is presented. The calibration curves were linear in the investigated areas, 1.25–10 mg/g of sulfadiazine and 0.25–2 mg/g trimethoprim. The recovery of sulfadiazine was 96–99%, and the recovery of trimethoprim 100–105%.
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