Abstract
A sensitive and validated method has been developed for simultaneous estimation of Naltrexone and Bupropion in pharmaceutical dosage form by using UV Spectroscopy, without prior separation, by four different techniques (Simultaneous Equation, Absorbance Ratio method, Dual Wavelength Method and Derivative Spectroscopic Method). The work was carried out on Shimadzu electron UV1800 double beam UV-Visible spectrophotometer. The absorption spectra of reference and test solutions were carried out in 1 cm matched quartz cell over the range of 200 - 400 nm. The linearity ranges for Naltrexone and Bupropion were 2-10 μg/ml. The results of the analysis have been validated statistically and by recovery studies. The proposed procedures are rapid, simple, require no preliminary separation steps and can be used for routine analysis of both drugs in quality control laboratories.
Highlights
Naltrexone (Figure 1) is (1S,5R,13R,17S)-4-(cyclopropylmethyl)-10,17-dihydroxy-12-oxa-4-aza pentacycl [9.6.1.01,13.05,17.07,18]octadeca-7(18),8,10-trien-14-one [1]
Literature survey reveals that some Spectrophotometric [5] and HPLC [4,5,6,7,8] methods have been reported for the estimation of Naltrexone and Bupropion in pharmaceutical formulations
Series of standard solutions of both Naltrexone and Bupropion in the concentration range of 2-10μg/ml were prepared in distilled water and the absorbances of these solutions were measured at 232 nm, 204 nm and 210 nm
Summary
Naltrexone (Figure 1) is (1S,5R,13R,17S)-4-(cyclopropylmethyl)-10,17-dihydroxy-12-oxa-4-aza pentacycl [9.6.1.01,13.05,17.07,18]octadeca-7(18),8,10-trien-14-one [1]. Bupropion (Figure 1) is 2-(tert-butylamino)-1-(3-chlorophenyl) propan-1-one [2]. It is used most commonly for the management of Major Depressive Disorder, Seasonal Affective Disorder and as an aid for smoking cessation. Literature survey reveals that some Spectrophotometric [5] and HPLC [4,5,6,7,8] methods have been reported for the estimation of Naltrexone and Bupropion in pharmaceutical formulations. The aim of this paper was to explore the possibility of using techniques of simultaneous equation method, dual wavelength method, isosbestic point method and derivative spectroscopic method for quantifying Naltrexone and Bupropion simultaneously in their mixture forms. Corresponding author E-mail address: Sai datri et al / World Journal of Biology Pharmacy and Health Sciences, 2020, 01(01), 033–041
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