Simultaneous Estimation of Metoprolol Succinate and Olmesartan Medoxomil in Pharmaceutical Dosage Form by RP-HPLC

Abstract

A simple, precise, specific and accurate reverse phase HPLC method has been developed for the simultaneous determination of Metoprolol succinate (METO) and Olmesartan medoxomil (OLME) in tablet dosage form. The chromatographic separation was achieved on inertsil ODS 3V (250 mm×4.6 mm, 5 μm) column using PDA detector. The mobile phase consisting of Buffer (2 ml O-phosphoric acid in 2000 ml milli-Q Water) and Acetonitrile in the ratio of 50:50 (v/v) at a flow rate of 1.0 mL/min was used. And the compounds were detected by a UV-detector at 220 nm at a column temperature of 25 ± 2°C. The retention time and drug content of Metoprolol succinate and Olmesartan medoxomil were 1.6 min, 100.2% and 2.4 min, 100.1%, respectively. The method was validated according to the ICH guidelines with respect to specificity, linearity (r2=0.999), accuracy (100 to 99.8%), precision and robustness (RSD<2%).