Abstract
A RP-HPLC method was developed (λ max = 280) to quantify hydroxychavicol and chlorogenic acid in Piper betel Linn. The method was validated for linearity, limit of detection (LOD = 3:1σ/S), limit of quantification (LOQ = 10:1σ/S), precision, accuracy and ruggedness. The response was linear with good correlation between concentration and mean peak area through a coefficient of determinants (r 2) of 0.9940, y = 1.98e + 004x + 5.19e + 004 and 0.9945, y = 2.76e + 004x + 1.40e + 005 with LOD 1.6 µg mL−1, 1.0 µg mL−1 and LOQ 5.0 µg mL−1 and 3.0 µg mL−1, respectively, for hydroxychavicol (28.56% w/w) and chlorogenic acid (0.40% w/w). The %RSD of precision and recovery of hydroxychavicol and chlorogenic acid were <2.0%. The proposed method was simple, accurate, specific, precise and reproducible.
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