Abstract
In this study, new and feasible UV-visible spectrophotometric and multivariate spectrophotometric methods were described for the simultaneous determination of hydrochlorothiazide (HCTZ), hydralazine hydrochloride (H.HCl), and reserpine (RES) in combined pharmaceutical tablets. Methanol was used as a solvent for analysis and the whole UV region was scanned from 200–400 nm. The resolution was obtained by using multivariate methods such as the net analyte signal method (NAS), principal component analysis (PCA), and net analyte signal-principal component analysis (NAS-PCA) applied to the UV spectra of the mixture. The results obtained from all of the three methods were compared. NAS-PCA showed a lot of resolved data as compared to NAS and PCA. Thus, the NAS-PCA technique is a combination of NAS and PCA methods which is advantageous to obtain the information from overlapping results.
Highlights
ObjectivesOur objective was to develop a new, rapid, and feasible simultaneous analytical UV/Vis spectrophotometric method combined with a multivariate calibration technique for the evaluation of HCTZ, H.HCl, and RES containing bulk drugs and combined tablet dosage forms because simultaneous estimation is less laborious, less time-consuming, and uses only a single solvent for analysis, which reduces the cost of chemicals
Hydrochlorothiazide, hydralazine hydrochloride, and reserpine are antihypertensive drugs used to lower blood pressure
I.e. net analyte signal method (NAS), principal component analysis (PCA), and net analyte signal-principal component analysis (NAS-PCA) were used to resolve the data of the ternary mixtures of HCTZ, H.HCl, and RES
Summary
Our objective was to develop a new, rapid, and feasible simultaneous analytical UV/Vis spectrophotometric method combined with a multivariate calibration technique for the evaluation of HCTZ, H.HCl, and RES containing bulk drugs and combined tablet dosage forms because simultaneous estimation is less laborious, less time-consuming, and uses only a single solvent for analysis, which reduces the cost of chemicals
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