Simultaneous Estimation of Curcumin and Vitamin E in Bulk and Cosmeceutical Formulation by UV Spectrophotometry

Abstract

The goal of present study is to validate the developed assay method per International Council for Harmonisation (ICH) Q2R1 recommendations and create a sensitive, reproducible, and practical method for detecting curcumin and Vitamin E in pure and in cosmeceutical formulations. With the creation and validation of a straightforward, accurate, and repeatable UV spectrophotometric method, curcumin and vitamin E in bulk and cosmeceutical formulation may now be determined simultaneously. The need for a new technique to estimate curcumin and vitamin E in a cosmeceutical formulation has become more pressing due to the lack of a well-described UV analytical method for doing so. Q absorption at 231 and 285 nm were used in the calculation. Vitamin E (16–24 μg/mL) and curcumin (8–12 μg/mL) both behave according to Beer-Lambert’s law at the designated wavelengths. The recovery experiments verified the method’s adherence to ICH standards for accuracy, precision, and resilience. The method under consideration can be employed to accurately determine the quantities of vitamin E and curcumin present in a cosmeceutical formulation