Abstract
An isocratic reversed-phase liquid chromatograpic assay method was developed for the quantitative determination of amlodipine besylate (AML) and indapamide (IND) in combined dosage form. A Brownlee C-18, 5 μm column with a mobile phase containing 0.02 M potassium dihydrogen phosphate–methanol (30+70, v/v) total pH-adjusted to 3 using o-phosphoric acid was used. The flow rate was 1.0 mL min−1 and effluents were monitored at 242 nm. The retention times of amlodipine besylate and indapamide were 5.9 min and 3.6 min, respectively. The proposed method was validated with respect to linearity, accuracy, precision, and robustness. The method was successfully applied to the estimation of amlodipine besylate and indapamide in combined tablet dosage forms.
Highlights
Amlodipine besylate (AML) is chemically 3-ethyl 5-methyl 2-[(2-aminoethoxy)methyl]-4-(2chlorophenyl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate benzenesulfonate
The mobile phase flow rate was maintained at Simultaneous Estimation of Amlodipine Besylate and Indapamide in Pharmaceutical Formulation by
The method was successfully applied for determination of amlodipine besylate (AML) and IND in its pharmaceutical formulations
Summary
The aim of the present study is to establish an accurate and sensitive RP-HPLC method and, after validation in accordance with International Conference on Harmonization (ICH) guidelines, to use the method for analysis of the drug content of both in tablet dosage form.[5]
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