SIMULTANEOUS ESTIMATION AND VALIDATION OF PARACETAMOL AND DOMPERIDONE IN BULK AND TABLET DOSAGE FORM BY USING DIFFERENT SPECTOPHOTOETRIC METHOD

Abstract

Three simple, accurate and precise spectrophotometric methods have been developed for simultaneous determination of Paracetamol and D omperidone in a binary mixture. In Method I absorbance we re measured at 258 nm and 292 nm corresponding to the absorbance maxima of Paracetamol and Domperidone. Concentration of each drug was obtained by using the absorptive values calculate d for both drugs at wavelengths 258 nm, and 292 nm. Method I I by Dual wa velength method, Paracetamol and Domperidone were quantified using principle that absorbance difference between two points on mixture spectra wa s directly proportional to concentration of component of interest and independent of interfering component. Meth od III describes Area under Curve and involves measurement of area un der curve in the range of 288 - 296 nm (For PARA) and 247 - 269 nm (For DOM) for the analysis in methanol. Linearity range was observed in the concentration range of 5 ‐ 30 μg/ml for Paracetamo l and Domperidone. Developed methods were applied to marketed formulation. The methods were validated statistically and recovery study was performed to confirm the accuracy of both methods. K eywords : Paracetamol, Domperidone, Ultraviolet spectroscopy, Simu ltaneous equation Method , Dual Wavelength Method, Area u nder Curve Method.