Abstract
Lignin with highly complex aromatic hydrocarbons is recalcitrant to biodegradation. Hydroxyl radical (OH) plays a vital role in lignin depolymerization, which can be generated from nanobubble water (NBW). This study focused on evaluating the effect of nitrogen gas NBW (N2-NBW) on lignin degradation and methane production via anaerobic co-digestion (AcoD) of waste activated sludge (WAS) and alkaline lignin. The results showed that total methane yield was obviously increased by 17% under N2-NBW addition, which followed a dose-dependent manner. Volatile fatty acids (VFAs) production and consumption in addition to volatile solids (VS) reduction were enhanced during the AcoD process supplemented with N2-NBW. Lignin degradation rate was improved by 10% under N2-NBW addition condition compared to the control at an initial lignin concentration of 50 mg/L. It's hypothesized that OH generated by NBW can facilitate lignin degradation and bioenergy conversion.
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