Simultaneous effects of potassium, rutin and temperature on performance of Manduca sexta caterpillars

Abstract

AbstractThe simultaneous effects on an insect herbivore (third instar tobacco hornworm Manduca sexta (L.): Sphingidae) of temperature (daytime temperatures of 20 °C, 25 °C and 30 °C), a mineral that may play a role in plant defense (potassium) and a common allelochemical (rutin) were examined in a factorial experiment. To manipulate potassium levels, a modified diet with limited plant material was used as the base and KCl and rutin added. Temperature affected efficiency of conversion of ingested food (ECI), efficiency of conversion of digested food (ECD), time to head‐capsule slippage, stadium duration, relative consumption rate (RCR) and relative growth rate (RGR) but not food consumed, biomass gained and approximate digestibility (AD). Potassium concentration influenced all of the variables except AD, time to head‐capsule slippage (HCS), duration of the stadium and percent of stadium time to HCS. Rutin impacted negatively on all of the variables except food consumed. Compared to larvae on the non‐rutin diets, fewer larvae fed rutin survived through molt initiation to ecdysis and fewer successfully completed ecdysis. Temperature and rutin had interactive effects for AD, ECD, RCR, RGR, time to HCS, and percent of stadium required to reach HCS. Rutin and potassium had interactive effects for biomass gained, RCR, ECI, time to HCS, duration of stadium, and percent of stadium required to reach HCS. Comparison of larval responses on an average potassium concentration (3.1%) versus high concentration (6.1%) showed that at the low daytime temperature increasing potassium concentration depressed biomass gained, but at the warmer temperatures potassium concentration had little effect unless rutin was present. In addition, potassium concentration had little impact on ECI unless rutin was present. These results indicate that significant interactive effects occur among temperature, potassium and rutin, and thus suggest that such interactive effects on larval performance may be common under field conditions, which are characterized by varying temperature and different concentrations of minerals and allelochemicals in hostplants.