Abstract
Hydrophilic interaction liquid chromatography coupled to mass spectrometry was employed for simultaneous determination of vitamin B6 and catechins in dietary supplements. The obtained results clearly shows the potential of the application of alcohol eluent (instead mainly used acetonitrile) as a component of an with zwitterionic stationary phase. The limits of detection on ZIC-HILIC column were 0.01 mg/L for catechins and vitamins B (only for pyridoxal phosphate was 0.10 mg/L). The investigations between green tea extract (GTE) and vitamin B were also evaluated using isobolographic analysis as well as the interaction indexes. Antioxidant activities of single components and their mixtures were determined by DPPH assay. It was found that the mixtures of GTE and vitamin B acted synergistically. In comparison to GTE alone, faster DPPH radical bleaching of the mixtures was observed in the presence of different forms of vitamin B6 (pyridoxine, pyridoxal, or pyridoxal phosphate), particularly for pyridoxal.
Highlights
Green tea is a worldwide consumed beverage owing to its health-promoting effect caused by the bioactive compounds, such as caffeine, amino acids, and several polyphenols [1, 2]
Vitamin B6 was determined in dietary supplements [20, 21], vegetables [22, 23], or in the urine samples [24] using C18 stationary phase
Pyridoxine was separated from other water-soluble vitamins using diol column in HILIC mode with 90:10 (w/v) ACN–water containing 5 mM ammonium acetate as mobile phase [25]
Summary
Green tea is a worldwide consumed beverage owing to its health-promoting effect caused by the bioactive compounds, such as caffeine, amino acids, and several polyphenols [1, 2]. Their popularity recently increased with scientific evidences that demonstrate its beneficial health effects, such as reduced risk of cardiovascular and degenerative diseases, likely due to the antioxidant properties of polyphenols [3, 4]. European Food Research and Technology (2020) 246:1609–1615 vitamins or minerals [12, 13] They have been used to cure nutritional deficiencies, weight loss, lowering cholesterol levels, and protecting skin from sun damage
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