Abstract
A quantitative gas-liquid chromatographic method has been developed for the simultaneous determination of the several monosaccharides present in glycosaminoglycans from animal tissues. In order to achieve a high degree of depolymerization of the glycosaminoglycans, it was found necessary to make them more susceptible to methanolysis by re- N-acetylation during the methanolysis procedure. Good resolution of all common monosaccharides, such as pertrimethylsilyl methyl glycosides, was achieved by the use of a capillary column of fused silica with the liquid phase CP tmSil 5. The method described was tested on glycosaminoglycans isolated from bovine periodontal ligament and the sensitivity (down to 3 μg monosaccharide) makes this method useful in the analysis of small amounts of soft connective tissues with low glycosaminoglycan contents.
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