Abstract
AbstractH‐point standard addition method (HPSAM) has been applied for simultaneous determination of tyrosine and histidine in trace levels using copper ions by adsorptive cathodic stripping voltammetry. The amino acids‐Cu(II) complexes were accumulated onto the surface of a hanging mercury drop electrode for 40 s. The reduction peaks of preconcentrated complexes were used for simultaneous determination of amino acids in the range 8.0–180 and 30–1100 nM for tyrosine and histidine respectively. The effect of various parameters such as pH, concentration of copper, accumulation time and scan rate on the selectivity were studied. Under the optimized conditions the method was successfully applied for determination of tyrosine and histidine in synthetic and real samples.
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