Abstract
A selective, simple and new reversed phase high performance liquid chromatographic method (HPLC) was developed and validated for simultaneous determination of tinidazole (TIN) and lidocaine (LID) in the ovule dosage form in this study. Developed method was performed with gradient elution by getting C18 (250x4.6mm,5µm) reversed phase HPLC column, mobile phase containing 10mM potassium phosphate buffer (pH3.0), acetonitrile at 1.0 mL/min. Temperatures for column and autosampler were adjusted at 25Cº. The chromatographic separation was carried out at 220 nm wavelength. Retention times were found as 6.5 min for LID and 8.2 min. for TIN. The purity of each substance was evaluated getting a photodiode array detector. The linearity ranges were 75.0-195.0 µg/mL for TIN and 25.0-65.0 µg/mL for LID. The limit of dedection (LOD) and the limit of quantitation (LOQ) results were 0.05625 µg/mL and 0.225 µg/mL for TIN, 0.01875 µg/mL and 0.075 µg/mL for LID, respectively. The simple, sensitive and reproducible method was applied for simultaneous determination of TIN and LID in pharmaceutical preparations successfully.
Highlights
The chemical name of TIN is 1-[2(ethylsulfonyl)ethyl]-2-methyl-5-nitro-1Himidazole and its closed formula is C8H13N3O4S
Tinidazole acts on Gardnerella vaginalis and most of the anaerobic bacteria (Bacteroides fragilis, Bacteroides melaninogenicus, Bacteroides spp., Clostridium spp., Eubacterium spp., Peptostreptococcus spp. and Veillonella spp.) with bacterial vaginosis treatment
The mechanism of action of TIN is still not clearly explained. It mediates the reduction of the nitro group and the low oxidation-reduction potential produced by the ferredoxin system and anaerobic bacteria only
Summary
The chemical name of TIN is 1-[2(ethylsulfonyl)ethyl]-2-methyl-5-nitro-1Himidazole and its closed formula is C8H13N3O4S. It was stated that it did not affect the rapid depolarization in phase 0, it was determined that these in vitro experiments were performed with the use of low K+ concentration and in the experiments performed by keeping the K+ concentration at a normal level, LID applied within the limits suitable for the therapeutic concentration decreased the ejection rate of the action potential. It does not change the action potential time in the atrium myocardium and shortens it considerably in the ventricular myocardium with Purkinje fibers. The developed method can be used in routine analyzes in the pharmaceutical industry
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