Abstract
In this study, a quantitative 1H NMR method (1H-qNMR) for determining the contents of imperatorin, byakangelicin, and oxypeucedanin in A. dahurica in traditional Chinese medicine (TCM) has been established. Dried plant material was extracted exhaustively with methanol by an ultrasonication-assisted extraction method. The 1H-qNMR measurements were performed on a 600 -MHz spectrometer with hydroquinone as the internal standard reference in deuterated dimethyl sulfoxide (DMSO-d6) solvent. Quantification was carried out using the 1H resonance signals at 6.55 ppm for hydroquinone and 7.68, 7.38-7.39, and 6.38-6.39 ppm for imperatorin, byakangelicin, and oxypeucedanin, respectively. The linearity, limit of detection (LOD), limit of quantitation (LOQ), precision, reproducibility, stability, and recovery of the methodology were evaluated, and results were good. The newly developed method has been applied to determine the three coumarins in A. dahurica.
Highlights
A. dahurica is a perennial medicinal plant belonging to the dry root of the umbelliferous plant A. dahurica or A. dahurica var. formosana [1]
0.932 e precision tests were performed by six replicate measurements of the reference standards with relative standard deviation (RSD) values considered as a measure of precision
Selection of Solvent and Internal Standard. e suitable deuterium-substituted solvent should have good solubility for the samples and the internal standard. e internal standard should have a sharp single peak, which is easy to recognize, and the spectrum peak should not overlap with the peak to be tested. rough the preliminary experiment, DMSO-d6 was selected as the solvent and hydroquinone was selected as the internal standard
Summary
A. dahurica is a perennial medicinal plant belonging to the dry root of the umbelliferous plant A. dahurica or A. dahurica var. formosana [1]. A. dahurica is warm, fragrant, spicy, and slightly bitter, which has the effect of relieving stuffy nose, dissipating cold, expelling wind and acesodyne, removing dampness, clearing swelling and excluding pus, spasmolysis, analgesia, relieving asthma, anti-inflammatory immunomodulation, and skin whitening. In clinical practice, it is widely applied in the treatment of common cold, headache, nasal obstruction, rhinorrhea, toothache, leucorrhea, acne and carbuncles, rheumatism, and especially for the headache caused by wind-cold invading Yang and Yin with obvious curative effect [3,4,5,6,7,8,9]. Isoimperatorin is a secondary plant metabolite that possesses multiple pharmacological properties, including fighting cancer-inducing substances, Relaxation delay (s) Integral area
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