Abstract
A rapid, sensitive, and accurate HPLC-DAD method was developed and validated for simultaneous determination of 10 stilbenes, which include suffruticosol A, suffruticosol B, suffruticosol C, resveratrol (E)-form, cis-ϵ-viniferin, trans-ϵ-viniferin, cis-suffruticosol D, cis-gnetin H, trans-suffruticosol D and gnetin H, in 44 plant samples from 9 different Paeonia species. In this optimized method, separations were carried out on an YMC-pack ODS-A column with water and methanol as mobile phase. The flow rate was set at 1.0 mL/min and the detector wavelength was set at 230 nm. The results showed that all the 10 analytes showed good linearity (r2 > 0.9994). The recoveries, measured at three concentration levels, varied from 97.19% to 102.89%. This method was also validated with respect to precision, repeatability, stability, and successfully applied to simultaneous determination of 10 stilbenes in 44 batches of peony seeds from 9 Paeonia species collected from different areas.
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