Abstract
Purpose: To develop an ultra-high performance liquid chromatography (UPLC) - photodiode array (PDA) method to compare the chemical composition of two different medicinal components of Pterocephalus hookeri.Methods: Samples were chromatographically separated in succession using Waters Acquity UPLCR BEH C18 column (2.1 × 100 mm, 1.7 μm) and gradient elution (0.2 % phosphoric acid aqueous -acetonitrile). Using partial least squares discriminant analysis and one-way analysis of variance, attempts were made to distinguish different medicinal parts of P. hookeri.Results: Regression equation for 10 compounds showed good linear regression (R2 > 0.9994). The relative standard deviations of precision, stability, repeatability and recovery were under 5 %. Compared with the aerial plant part, the root had significantly higher levels of sylvestroside I (p < 0.01), cantleyoside (p < 0.001), dipsanosides B (p < 0.01) and dipsanosides A (p < 0.01), but significantly lower levels of loganic acid (p < 0.001), chlorogenic acid (p < 0.01), and isochlorogenic acid (p < 0.01). There were no significant differences between loganin, sweroside and isochlorogenic acid C.Conclusion: The described method is simple, accurate and reproducible, and can be used for the simultaneous determination of 10 major compounds of P. hookeri. The results demonstrate that there is variation in the chemical composition of the aerialpart and root of P. hookeri and that loganic acid and cantleyoside are the primary chemical biomarkers.Keywords: Tibetan medicine, Pterocephalus hookeri, Medicinal parts, Loganic acid and Cantleyoside, UPLC-PDA
Highlights
Pterocephali herba is the whole herb of the perennial plant Pterocephalus hookeri
In Tibetan, P. hookeri is called “Bangzi-du-wu,” and it is recorded as a Tibetan medical classic named “rGyud-bZhi” [2,3] in “Drug Standards of Tibetan Medicines” [4] and the 2015 edition of the Chinese Pharmacopoeia [5]
Recent studies indicate that the main effective components of P. hookeri are glycosides, including iridoid glycosides and triterpenoid saponins [2,7,8]
Summary
Pterocephali herba is the whole herb of the perennial plant Pterocephalus hookeri (C.B. Clarke) Höeck, a member of the Dipsacaceae family [1]. As a traditional national medicine, P. hookeri has been widely used in many Tibetan herbal medicine formulations and is clinically used to treat diseases including common cold, rheumatoid arthritis, enteritis, dysentery, and other conditions [6,7]. Recent studies indicate that the main effective components of P. hookeri are glycosides, including iridoid glycosides (loganin and cantleyoside) and triterpenoid saponins (hookerosides A-D) [2,7,8]. Several analytical approaches including highperformance liquid chromatography (HPLC) [10], capillary zone electrophoresis [11], and ultra-fast liquid chromatography (UFLC) [12] have been used to quantify one compound or simultaneously quantify several compounds in P. hookeri. As far as we know, there is no publication describing the use of PLS-DA and simultaneous quantitation of 10 compounds to distinguish between different medicinal parts of P. hookeri
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