Abstract
A thermospray high-performance liquid chromatographic-mass spectrometric (LC-MS) method has been developed for the analysis of sulfonamides such as sulfachlorpyridazine, sulfadiazine, sulfadimethoxine, sulfadimidine, sulfadoxine, sulfamerazine, sulfamethoxazole, sulfamethoxypyridazine, sulfamonomethoxine, sulfaquinoxaline, sulfathiazole and sulfisomidine. The LC separation was carried out on a TSK-gel ODS-80TM by using 0.05 M ammonium acetate (pH 4.5)- acetonitrile (7 : 3) as the mobile phase, at a flow rate of 0.8 ml/min. The vaporizer temperature was set to 155°C and ion source block temperature to 280°C. The mass spectra obtained from sulfonamides were very simple, with base peaks corresponding to protonated molecular ions MH+. The calibration curve for sulfadimidine was rectilinear from 0.2 to 20 ng with the detection limit by selected ion monitoring (SIM) was about 100 pg. Similar results were obtained with other sulfonamides. An excellent correlation between the results of the thermospray LC-MS method and HPLC method was obtained (γ=0.981).
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