Simultaneous Determination of Some Analgesic Drugs Using Mixed Mode Stationary Phase

Abstract

A new HPLC method based on a mixed mode stationary phase Hypersil Duet C18/SAX was developed and applied for the simultaneous determination of acetaminophen, acetylsalicylic acid and codeine. Parameters, such as the composition of the mobile phase, the nature of the organic modifier, the buffer type and the flow rate were investigated to optimize the separation. The results obtained show that the new HPLC method is rapid, highly efficient and selective. The studied compounds are separated in 10 min, by means of a mobile phase containing phosphate buffer (pH 7.50) and methanol (65:35 v v−1). The retention mechanisms of each analyte were investigated using both the linear solvent strength theory and stoichiometric displacement model. The method was fully validated and showed good linearity for each compound for a concentration ranging between 2.0 and 40 μg mL−1. The limits of detection and quantification were determined and they are lower than 0.1 μg mL−1. The precision (RSD) of the method does not exceed 2 % for all studied compounds. The method was successfully applied for the assay of acetaminophen, acetylsalicylic acid and codeine in pharmaceutical formulations.