Abstract
ObjectiveThe inadvertent combination of sildenafil (SLD) and nitric oxide releasing compounds (NRC) may cause a life threatening hypotension and conversion of coital angina into an irreversible one. The aim of this study was to develop and validate a UPLC MS/MS method for the simultaneous quantitative analysis of SLD, nicorandil (NRD), and ARG in human plasma to determine the safety margins for drug combinations. Design and methodChromatographic elution was achieved in 4min using gradient elution and an injection volume of 10μL. Electro-spray positive ionization (ESI+ve) detection and multiple-reaction monitoring mode (MRM) were used for detection. ResultsThe method was found to be linear (10–900ng/mL for SLD and NRD while 1–30μg/mL for ARG), accurate and precise (99.35±1.58, 99.62±1.13, and 100.04±1.22% for SLD, NRD and ARG; respectively) and met all other validation requirements. ConclusionThe developed UPLC MS/MS method is suitable for fast, sensitive, accurate and simultaneous determinations of SLD, NRD, and ARG in plasma.
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