Simultaneous determination of seven effective constituents in the leaves of bamboo from different species of phyllostachys by HPLC

Abstract

A high performance liquid chromatography (HPLC) method was established for determining contents of seven effective constituents (orientin, isoorientin, vitexin, isovitexin,chlorogenic acid, caffeic acid and ferulic acid) in Bamboo leaf from different provenances of Phyllostachys. Seven effective constituents were simultaneously determined by HPLC with Sunfire C18 ODS column ( 4.6 mm×250 mm, 5µm ) by gradient elution using acetonitrile (A) and 1.0% acetic acid (B) as the mobile phase. The flow rate was 1.0 mL· min−1; the detection wavelength was 335 nm with column temperature at 25 °C. Seven effective constituents were separated clearly and respectively in 35 min, the linear ranges of orientin, isoorientin, vitexin, isovitexin, chlorogenic acid, caffeic acid and ferulic acid was 0.093–0.623 µg(r=0.9994), 0.058–0.385 µg(r=0.9994), 0.045–0.301 µg(r=0.9987), 0.041–0.272 µg(r=0.9992), 0.059–0.396 µg(r=0.9997), 0.046–0.312 µg(r=0.9992), 0.103–0.684 µg(r=0.9983), respectively; the average recoveries (n=3) were 98.62%, 98.60%, 100.64%, 99.52%, 99.56%, 97.56% and 101.74% respectively. The method is simple, accurate and can be used for quality control and evaluation of different provenances of Phyllostachys.