Abstract
The liquid chromatography method for simultaneous determination of seven carbamate pesticides, viz. aldicarb, benomyl, carbofuran, ethiofencarb, methomyl, thiophanate ethyl and thiophanate methyl has been described. The method employed a Zorbax Eclipsed XGB C-8, column and 30:70 acetonitrile–water mixture (v/v) as mobile phase with UV detection at 235nm. Good separation of all these carbamates was achieved at 1mL/min flow rate of the mobile phase at 25°C. Their recovery from food grains fenugreek leaves and apples was found to be >80%. The commercial grade carbamate formulations containing benomyl, carbofuran, methomyl and thiophanate methyl were also analyzed and residue levels of these commercial formulations on selected food matrices were analyzed for comparison. The method had good peak resolution, high precision, low limit of detection and high degree of reproducibility. It could be applied for rapid screening and quantization of these carbamates in real sample matrices sold in the local markets.
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