Abstract
A novel method based on high performance liquid chromatography with variable wavelength detection was developed for quantitative analysis of the bioactive ingredients in a Chinese herbal preparation called Kangnaoshuai capsule (KNS). Seven bioactive ingredients, namely puerarin, albiflorin, stilbene glucoside, salvianolic acid B, baicalin, baicalein, and wogonin were simultaneously determined. The separation was carried out on a RP-C18 column with acetonitrile and 0.1% phosphoric acid as mobile phase under gradient conditions. Five detection wavelengths, 248 nm for puerarin, 230 nm for albiflorin, 320 nm for stilbene glucoside, 275 nm for baicalin, baicalein and wogonin, and 286 nm for salvianolic acid B, were utilized during a single elution process. The validation data proved the developed method to be sensitive, precise, accurate, and selective, and was successfully applied for the determination of commercial KNS samples from different production batches and different manufacturers.
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