Simultaneous Determination of Seven Active Components in Rat Plasma by UHPLC-MS/MS and Application to a Quantitative Study after Oral Administration of Huang-Lian Jie-Du Decoction in High Fat-Induced Atherosclerosis Rats.

Guoliang Xu,Qiyun Zhang,Peng Nie,Yanling Xiong,Lanbin Yu,Xiaojun Yan,Li Jiang,Yu Chen,Xue Ding

doi:10.1155/2019/5628160

Abstract

Huang-Lian Jie-Du decoction (HLJDD) has been used to treat cardiovascular and cerebrovascular disease for many years in China. Currently, the determination of effect components in HLJDD is focusing either on the formula or on the extract, while quantification of that in biological samples is scarce, especially simultaneous determination of multicomponent. In this paper, a rapid, specific, and sensitive ultra-high performance liquid chromatography-tandem mass spectrometry method was developed and fully validated for the simultaneous determination of seven main active constituents, i.e., baicalin, baicalein, wogonoside, wogonin, berberine, palmatine, jatrorrhizine in rat plasma. The method was also successfully applied to a quantitative study after oral administration of HLJDD at different doses of 1.5, 3, and 6 g/kg body weight to high fat-induced atherosclerosis rats. The analytes were detected by ESI source and multiple reactions monitoring (MRM) using positive scanning mode. The blood was collected from the abdominal aorta of rats at predetermined time and preprepared with icariin and tetrahydropalmatine as internal standards (IS). Sample preparation was achieved by protein precipitation (PPT). The validation parameters (linearity, sensitivity, intra-/interday precision and accuracy, extraction recovery, and matrix effect) were within acceptable ranges, and biological extracts were stable during the entire storing and preparing process. And the result of determination of HLJDD-containing plasma, baicalin, baicalein, wogonoside, and wogonin could be highly detected in a dose-dependent manner while berberine, jatrorrhizine, and palmatine were determined in a very low level and in a dose-independent mode. Thus, the established method was sensitive enough and successfully applied to the determination of seven effective components in plasma taken from 24 high fat-induced atherosclerosis rats after oral administration of three dosages of HLJDD.

Highlights

Atherosclerosis (AS) is a chronic disease with a typical pathological process of abnormal lipid metabolism, i.e., infiltrating into the arterial intima and depositing on the vessel wall which triggers inflammation and in turn leads to the abnormal response to injury of the vessel wall [1], such as alteration, exudation, and proliferation
International Journal of Analytical Chemistry the pathogenesis of AS is not fully understood, inflammationinjury-reaction theory proposed by Professor Ross [3] has been widely recognized around the world
From the result of determination of Huang-Lian Jie-Du decoction (HLJDD)-containing plasma, baicalin, baicalein, wogonoside, and wogonin could be highly detected in a dose-dependent manner while berberine, jatrorrhizine, and palmatine were determined in a very low level and in a dose-independent mode

Summary

Introduction

Atherosclerosis (AS) is a chronic disease with a typical pathological process of abnormal lipid metabolism, i.e., infiltrating into the arterial intima and depositing on the vessel wall which triggers inflammation and in turn leads to the abnormal response to injury of the vessel wall [1], such as alteration, exudation, and proliferation. In TCM, there are many theories according to the pathogenesis of AS, such as damp heat (Shi Re in Chinese), toxic pathogen (Du Xie in Chinese), and phlegm stasis (Tan Yu in Chinese); that is to say, therapeutic principles of clearing away heat and dampness and detoxicating would be effective for AS

Results

Discussion

Conclusion