Abstract
Anti-nutritional components such as: oxalates, thiocyanates and phytic acid were determined in 20 plants samples originated from Traditional Chinese Medicine and Ayurveda (e.g., Andrographis paniculata, Angelica sinensis, Emblica officinalis, Withania somnifera, Polygonum multiflorum, Bacopa monnieri, Pueraria lobata, Cola accuminata, Curcuma longa, Ocimum sanctum, Mucuna pruriens, Garcinia cambogia, Panax ginseng, Bupleurum sinensis, Salviae miltiorrhizae, Schisandra sinensis, Terminalia arjuna). Extraction and analysis procedures for these anti-nutritional components determination were modified and optimized. Water-soluble oxalates, thiocyanates and phytic acid were simultaneously determined by new procedure with ion chromatography method, resulting in analyzed samples: 0.38–145.39 mg 100 g−1 dry mass (d.m.) for oxalates, 0.94–34.34 mg 100 g−1 d.m. for thiocyanates, whereas 0.42–16.13 mg 100 g−1 d.m. for phytic acid. The highest of the total content of anti-nutritional components was observed for the O. sanctum, but the lowest for C. longa. Moreover, oxalates and phytic acid were determined by titration, whereas thiocyanates by spectrophotometric method.
Highlights
Traditional Chinese Medicine and Ayurveda are using a number of plants for treatment of different diseases
Ashwagandha, Amla, A. paniculata, A. sinensis (AS), Bacopa monnieri (BM), Bupleurum sinensis (BS), Cola accuminata (CA), Curcuma longa (CL), FoTi, Garcinia cambogia (GC), Mucuna pruriens (MP), Ocimum sanctum (OS), Panax ginseng (PG), Pueraria lobata (PL), Salvia miltiorrhizae (SM), Schisandra sinensis (SSS), Terminalia arjuna (TA) were purchased from Standard (Lublin, Poland), while A. paniculata APl-B was supplied from Biofaktor (Gorzów Wlkp., Poland) and Schisandra sinensis coded as SS-FL from FENG-LUKA (Goleniów, Polska).Oxalic acid (H2C2O4 × 2H2O), phytic acid, potassium thiocyanate (KSCN) standard and trichloroacetic acid (TCA) were purchased from Sigma-Aldrich (Poland)
Thiocyanates and phytic acid in the tested plant materials were determined by ion chromatography method using modified procedure
Summary
Traditional Chinese Medicine and Ayurveda are using a number of plants for treatment of different diseases. Considering to the above properties and because of in the literature there is a lack of information on the studied substances content in plants, it is necessary to determine the concentration level of oxalates, thiocyanates and phytates in the popular medicinal plants, herbs and dietary supplements. For this purpose, proposed, new and optimized methodology [ion chromatography (IC) technique] for simultaneous determination of studied anti-nutritional compounds has been applied. Oxalates and phytic acid were determined by titration, whereas thiocyanates by spectrophotometric method
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