Abstract
Partial least squares regression (PLSR) was used for the simultaneous quantification of rifampicin (RIF)and isoniazid (INH) by visible spectrophotometry using a simple derivatization reaction. In the presence of neocuproine, copper(II) is easily reduced by INH to a Cu(I)-neocuproine complex that shows an absorption maximum at 455 nm. Under these conditions, RIF shows an absorption maximum at 449 nm. The calibration set was established between 8and 57 mg L-1 for RIFand 1.5and 7 mg L-1 for INH. The method was applied to the determination of the drugs in urine samples (recoveries between 92and 119%)and in pharmaceutical formulations (relative error lower than 5%).
Highlights
The World Health Organization (WHO) classifies tuberculosis (TB) as a neglected disease that affects thousands of people but does not present an attractive opportunity for economic investment and development of pharmaceuticals, for reaching people in developing countries.[1]
One of the most effective antituberculosis treatments used in many countries is based on a fixed dose combination (FDC) of two or more tuberculostatic agents in a single pharmaceutical formulation
The analytical methods include chromatographic techniques such as high-performance thin-layer chromatography (HPTLC),[6] high-performance liquid chromatography (HPLC),[7,8,9,10,11,12] ultra-performance liquid chromatography (UPLC),[13] micellar electrokinetic capillary chromatography (MEKC),[14] and, less frequently, spectrophotometric analysis combined with multivariate regression,[15,16,17] derivative spectrophotometry[18] and voltammetric methods.[19,20,21]
Summary
The World Health Organization (WHO) classifies tuberculosis (TB) as a neglected disease that affects thousands of people but does not present an attractive opportunity for economic investment and development of pharmaceuticals, for reaching people in developing countries.[1]. One of the most effective antituberculosis treatments used in many countries is based on a fixed dose combination (FDC) of two or more tuberculostatic agents in a single pharmaceutical formulation. The analysis of antituberculosis drugs (e.g., rifampicin, isoniazid, pyrazinamid and daptomycin) has been performed for pharmaceutical formulations and/or biological fluids. The analytical methods include chromatographic techniques such as high-performance thin-layer chromatography (HPTLC),[6] high-performance liquid chromatography (HPLC),[7,8,9,10,11,12] ultra-performance liquid chromatography (UPLC),[13] micellar electrokinetic capillary chromatography (MEKC),[14] and, less frequently, spectrophotometric analysis combined with multivariate regression,[15,16,17] derivative spectrophotometry[18] and voltammetric methods.[19,20,21]
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